Figure 6

Lower levels of UCH-L1 decrease phosphorylation of MLC2 in H157 cells. A. Immunoblot of pMLC-2 protein, total MLC2, UCH-L1 knockdown and β-actin loading control in H157 cells post siRNA treatment. B. Densitometry analysis for 3 sets of blots exhibiting UCH-L1 protein level in untreated H157 cells and cells treated with either scrambled siRNA or UCH-L1 siRNA. UCH-L1 protein levels in H157 cells were normalized to β-actin. C. Densitometry analysis for 3 sets of blots exhibiting MLC2 phosphorylation in untreated H157 cells and cells treated with either scrambled siRNA or UCH-L1 siRNA. Phospho-MLC2 protein levels in H157 cells were normalized to β-actin.