Factors that contribute to long-term survival in patients with leukemia not in remission at allogeneic hematopoietic cell transplantation
- Hideo Koh1,
- Hirohisa Nakamae1Email author,
- Kiyoyuki Hagihara1,
- Takahiko Nakane1,
- Masahiro Manabe1,
- Yoshiki Hayashi1,
- Mitsutaka Nishimoto1,
- Yukari Umemoto1,
- Mika Nakamae1,
- Asao Hirose1,
- Eri Inoue1,
- Atsushi Inoue1,
- Masahiro Yoshida1,
- Masato Bingo1,
- Hiroshi Okamura1,
- Ran Aimoto1,
- Mizuki Aimoto1,
- Yoshiki Terada1,
- Ki-Ryang Koh1,
- Takahisa Yamane1,
- Masahiko Ohsawa2 and
- Masayuki Hino1
© Koh et al; licensee BioMed Central Ltd. 2011
Received: 13 January 2011
Accepted: 10 April 2011
Published: 10 April 2011
There has been insufficient examination of the factors affecting long-term survival of more than 5 years in patients with leukemia that is not in remission at transplantation.
We retrospectively analyzed leukemia not in remission at allogeneic hematopoietic cell transplantation (allo-HCT) performed at our institution between January 1999 and July 2009. Forty-two patients with a median age of 39 years received intensified conditioning (n = 9), standard (n = 12) or reduced-intensity conditioning (n = 21) for allo-HCT. Fourteen patients received individual chemotherapy for cytoreduction during the three weeks prior to reduced-intensity conditioning. Diagnoses comprised acute leukemia (n = 29), chronic myeloid leukemia-accelerated phase (n = 2), myelodysplastic syndrome/acute myeloid leukemia (MDS/AML) (n = 10) and plasma cell leukemia (n = 1). In those with acute leukemia, cytogenetic abnormalities were intermediate (44%) or poor (56%). The median number of blast cells in bone marrow (BM) was 26.0% (range; 0.2-100) before the start of chemotherapy for allo-HCT. Six patients had leukemic involvement of the central nervous system. Stem cell sources were related BM (7%), related peripheral blood (31%), unrelated BM (48%) and unrelated cord blood (CB) (14%).
Engraftment was achieved in 33 (79%) of 42 patients. Median time to engraftment was 17 days (range: 9-32). At five years, the cumulative probabilities of acute graft-versus-host disease (GVHD) and chronic GVHD were 63% and 37%, respectively. With a median follow-up of 85 months for surviving patients, the five-year Kaplan-Meier estimates of leukemia-free survival rate and overall survival (OS) were 17% and 19%, respectively. At five years, the cumulative probability of non-relapse mortality was 38%. In the univariable analyses of the influence of pre-transplant variables on OS, poor-risk cytogenetics, number of BM blasts (>26%), MDS overt AML and CB as stem cell source were significantly associated with worse prognosis (p = .03, p = .01, p = .02 and p < .001, respectively). In addition, based on a landmark analysis at 6 months post-transplant, the five-year Kaplan-Meier estimates of OS in patients with and without prior history of chronic GVHD were 64% and 17% (p = .022), respectively.
Graft-versus-leukemia effects possibly mediated by chronic GVHD may have played a crucial role in long-term survival in, or cure of active leukemia.
Patients with primary refractory or refractory relapsed acute leukemia have an extremely poor prognosis. It has been generally recognized that few cases with primary refractory or refractory relapsed acute leukemia can be cured using conventional chemotherapy alone . While allogeneic hematopoietic cell transplantation (allo-HCT) has the potential to cure even active leukemia, it has not been determined what subgroup can receive a long-term benefit from it.
Several retrospective studies have reported the prognostic factors for allo-HCT in patients not in remission at allo-HCT including untreated first relapse cases [2–8]. However, the factors contributing to long-term survival have not been established because the follow-up periods of these studies were not long enough at less than five years. Importantly, it can be assumed that patients who survive for more than five years without leukemia relapse are most likely cured. Only one large-scale retrospective study has examined long-term outcomes for more than five years following allo-HCT in adult patients with acute leukemia not in remission . This study showed that several pre-transplant variables including complete remission duration, type of donor, disease burden, performance status, age and cytogenetics affected survival. However, whether post-transplant variables such as acute or chronic graft-versus-host disease (GVHD) influenced the post-HCT prognosis was not assessed. To our knowledge, no studies have investigated pre- and/or post-transplant factors which are associated with long-term survival exclusively in adult patients with active leukemia at allo-HCT. Therefore, we comprehensively evaluated the pre- and post-transplant factors which contribute to long-term survival of more than five years in patients with leukemia not in remission at allo-HCT.
Patients and methods
Between January 1999 and July 2009, 42 consecutive patients (24 males and 18 females) with leukemia not in remission, aged 15 to 67 years (median age: 39 years), underwent allo-HCT at our institution. Patients with de novo acute myeloid leukemia (AML; n = 17), acute lymphoblastic leukemia (ALL; n = 12), chronic myeloid leukemia in accelerated phase (CML-AP; n = 2), myelodysplastic syndrome (MDS) overt AML (n = 10) and plasma cell leukemia (n = 1) were included. High-risk AML was defined according to the Eastern Cooperative Oncology Group/Southwest Oncology Group classification as having poor-risk cytogenetics (5/del[5q], 7/del[7q], inv[3q], abn11q, 20q or 21q, del[9q], t[6;9], t[9;22], abn17p, and complex karyotype defined as three or more abnormalities) . High-risk ALL was defined as having poor-risk cytogenetics with either t(4:11), t(9;22), t(8;14), hypodiploidy or near triploidy, or more than five cytogenetic abnormalities . Of study subjects with acute leukemia, cytogenetic abnormalities were intermediate (n = 17, 44%) or poor (n = 22, 56%). Seven patients were primary refractory to induction chemotherapy. The other patients relapsed after conventional chemotherapy (n = 23) or the first or the second HCT (n = 9). The median number of blast cells in bone marrow (BM) was 26.0% (range; 0.2-100) before the start of chemotherapy for allo-HCT. Six patients had leukemic involvement of the central nervous system (CNS). Stem cell sources were related BM (n = 3, 7%), related peripheral blood (PB) (n = 13, 31%), unrelated BM (n = 20, 48%) and unrelated cord blood (CB) (n = 6, 14%). Standard serologic typing was used for human leukocyte antigen (HLA) -A, B and DRB1. Thirty-one pairs were matched for HLA-A, B and DRB1 antigens. Three patients were mismatched for one HLA antigen (two at HLA-A, one at HLA-B), and seven were mismatched for two (two at HLA-A and B, five (all CB) at HLA-B and DRB1). The remaining one patient was mismatched for all three antigens (haploidentical). We classified conditioning regimens into four categories. Standard conditioning (n = 12) comprised a busulfan-based or total body irradiation (TBI)-based (12Gy) regimen. Busulfan was given as a total of 16 mg/kg orally or equivalent dose, 12.8 mg/kg intravenously (i.v.). Intensified conditioning (n = 9) consisted of additional cytoreductive chemotherapy in the three weeks before conditioning, followed by standard conditioning. Of the 21 patients receiving standard or intensified conditioning, 13 patients received the TBI-based regimen. Reduced-intensity conditioning (n = 21) comprised a fludarabine-based (n = 20) and cladribine-based regimen (n = 1). Fludarabine was given as 25-35 mg/m2 i.v. on five or six consecutive days. Of the 21 patients receiving reduced-intensity conditioning, 14 patients received cytoreductive chemotherapy in the three weeks before conditioning. Prophylaxis for acute GVHD was a calcineurin inhibitor alone (n = 5), calcineurin inhibitor plus short-term methotrexate (n = 32), calcineurin inhibitor plus mycophenolate mofetil (n = 2), or none (n = 3). The calcineurin inhibitor included cyclosporine administered to 33 patients and tacrolimus to six patients.
The absence of post-transplant remission in some patients biased the calculation of relapse rate, nonrelapse mortality (NRM) and leukemia-free survival (LFS). Therefore, we set five-year overall survival (OS) as the primary end point. OS was defined as time from the date of last transplantation to the date of death or last follow-up. LFS was defined as time from the date of last transplantation to the date of disease relapse, death during remission or last follow-up. NRM was defined as a death not related to disease. Neutrophil recovery was defined as an absolute neutrophil count of at least 500 cells/mm3 for three consecutive time points. Platelet recovery was defined as a count of at least 20 000 platelets/mm3 without transfusion support. Acute GVHD (aGVHD) was defined in accordance with standard criteria . Chronic GVHD (cGVHD) was evaluated in patients surviving for more than 100 days after allo-HCT and was classified into limited or extensive type .
If the disease for which the patient underwent transplantation was present at the time of death or found at autopsy, we defined disease relapse/progression as the primary cause of death. Unadjusted survival probabilities were estimated using the Kaplan and Meier method and compared using the log-rank tests. Cumulative incidence curves were used in a competing-risks model to calculate the probability of aGVHD, cGVHD and NRM . For neutrophil and platelet recovery, death before neutrophil or platelet recovery was the competing event; for GVHD, death without GVHD and relapse were the competing events; and, for NRM, relapse was the competing event. In order to examine the impact of cGVHD on survival, we performed a landmark analysis, which divided patients according to their prior history of cGVHD at 6 months post-transplant . We excluded from landmark analysis patients who died or relapsed less than 6 months after transplant, and did not use the information on whether or not patients developed cGVHD 6 months after transplant. Multivariable analysis of prognostic factors for the primary outcome could not be conducted due to lack of statistical power. Instead, we performed a landmark analysis, which divided patients according to the significant pre-transplant factors and their prior history of cGVHD at 6 months post-transplant. All P values were 2-tailed and considered statistically significant if the values were less than 0.05. All statistical analyses were performed using the PASW Statistics17.0 (SPSS Inc, Chicago, IL, USA) and the statistical software environment R, version 2.9.1.
Baseline characteristics of study participants
de novo AML
MDS overt AML
Status at allo-HCT
Primary refractory/Refractory relapse/Untreated MDS overt AML
No. chemo regimens prior allo-HCT
Time from diagnosis to allo-HCT (days)
Marrow blasts at allo-HCT
Reduced-intensity + cytoreductive chemotherapy
Calcineurin inhibitor alone
Calcineurin inhibitor + sMTX
Calcineurin inhibitor + MMF
Donor (HLA-A, B and DRB1 antigens)
Matched related PB/BM
Mismatched related PB/BM
Matched unrelated BM
Mismatched unrelated BM
Umbilical cord blood
Neutrophil engraftment was achieved in 33 (79%) of 42 patients. The median time to neutrophil engraftment was 17 days (range, 9-32). In a total of four of 27 evaluable patients, a platelet count > 20 000/μl was not achieved. In the patients that achieved platelet counts of ≥ 20 000/μl, the median time to platelet engraftment was 33 days (range, 13-99). The cumulative probabilities of neutrophil and platelet engraftment were 79% and 55%, respectively.
Twenty-four of 42 patients developed aGVHD (eight grade I, nine grade II, five grade III, two grade IV). Twelve of 24 evaluable patients developed cGVHD (one limited, 11 extensive). At five years, the cumulative probabilities of aGVHD and cGVHD were 63% and 37%, respectively.
A total of eight patients were alive at the time of this analysis, seven in complete remission (CR). The most common cause of death was disease relapse/progression. Causes of death were disease relapse/progression (n = 27), GVHD (n = 2), sinusoidal obstruction syndrome (SOS) (n = 3), Epstein-Barr virus associated post-transplant lymphoproliferative disorder (n = 1), and adenovirus infection (n = 1). Of six patients with CNS lesion, five died of disease relapse/progression (n = 3), GVHD (n = 1) and SOS (n = 1), and one was alive at last follow-up although another HCT was planned due to BM relapse post-transplant. At five years, the cumulative probability of NRM was 38%. Nine patients died before day 30, and 18 patients died within the first 100 days post-HCT.
LFS and OS
A total of 22 of 33 evaluable patients attained a CR after the allo-HCT. The median follow-up of survivors was 85 months (range, 24-126 months). The five-year Kaplan-Meier estimates of LFS and OS were 17% and 19%, respectively.
Univariable analysis of impact of pre-transplant variables on overall survival
Survival (% at 5 y)
Log rank P value
Age at allo-HCT
MDS overt AML
Marrow blasts at allo-HCT
Umbilical cord blood
Reduced-intensity + cytoreductive chemotherapy
Our data showed that allo-HCT resulted in long-term disease remission and an eventual cure of active leukemia in a subset of de novo AML or ALL patients with marrow blast ≤ 26% and without poor-risk cytogenetics, possibly by graft-versus-leukemia (GVL) effects mediated through cGVHD.
A retrospective study with a large cohort using data reported to the Center for International Blood and Marrow Transplant Research demonstrated that pre-transplant variables delineated subgroups with different long-term allo-HCT outcomes in adult patients with acute leukemia not in remission . However, they did not address the effect of cGVHD on survival. Baron et al. have reported that extensive cGVHD was associated with decreased risk of progression or relapse in patients with AML or MDS in complete remission at the time of nonmyeloablative HCT . However, it remains unclear whether cGVHD is associated with long-term disease control in patients who have active leukemia at transplant. The results of the current study showed that GVL effects mediated by cGVHD may play a crucial role in long-term survival in or a cure of active leukemia, especially in patients without poor-risk cytogenetics. Further study on the possible relationship between cGVHD and GVL effects would be very helpful in the management of immunosuppressive treatment.
For patients who were ineligible for myeloablative conditioning due to comorbidities coupled with rapidly progressive leukemia, we administered sequential cytoreductive chemotherapy, followed by reduced-intensity conditioning for allo-HCT in order to reduce toxicity and obtain sufficient anti-leukemic efficacy. The utility of the combination of sequential cytoreductive chemotherapy and reduced-intensity conditioning for allo-HCT was previously reported . Our results did not show that this sequential regimen had an advantage in controlling active leukemia. However, we speculated that effective tumor reduction by individual chemotherapy and/or conditioning for allo-HCT to control disease until cGVHD subsequently occurred might also be important, particularly in rapidly proliferating leukemia. In contrast, intensive conditioning did not appear to be essential in relatively indolent leukemia, even with non-remission.
Based on our results, CB might be unsuitable as a source of stem cells for treatment of active leukemia at the time of allo-HCT. However, most patients receiving CBT could not wait for an unrelated donor search because their disease tended to be aggressive compared with those in the unrelated BM group. Thus, it is difficult to arrive at any conclusions about the best stem cell source for allo-HCT in patients in non-remission status based solely on our results.
Our study has several limitations. The results might be affected by an underlying selection bias due to the nature of retrospective data. Also, our study was limited by the small number of patients, the heterogeneity of the disease, the transplant procedure and the stem cell source. However, the major strengths of our study were that the follow-up period was sufficient with more than 5 years and the impact of cGVHD as well as pre-transplant factors on long-term survival were analyzed exclusively for subjects with active leukemia.
These data show that allo-HCT has the potential to cure active leukemia possibly via cGVHD, particularly in patients with favorable factors even when in non-remission. Further research is warranted to explore the essential factors contributing to the success of allo-HCT such as intensity of conditioning, and GVL effects mediated through cGVHD.
This work was supported by a Grant-in-Aid for Scientific Research from the Japanese Ministry of Education, Science, Sports, and Culture, and a grant from the Japanese Ministry of Health, Welfare, and Labour.
- Champlin R, Gale RP: Acute myelogenous leukemia: recent advances in therapy. Blood. 1987, 69: 1551-1562.PubMedGoogle Scholar
- Biggs JC, Horowitz MM, Gale RP, Ash RC, Atkinson K, Helbig W, Jacobsen N, Phillips GL, Rimm AA, Ringdén O, et al.: Bone marrow transplants may cure patients with acute leukemia never achieving remission with chemotherapy. Blood. 1992, 80: 1090-1093.PubMedGoogle Scholar
- Sierra J, Storer B, Hansen JA, Bjerke JW, Martin PJ, Petersdorf EW, Appelbaum FR, Bryant E, Chauncey TR, Sale G, et al.: Transplantation of marrow cells from unrelated donors for treatment of high-risk acute leukemia: the effect of leukemic burden, donor HLA-matching, and marrow cell dose. Blood. 1997, 89: 4226-4235.PubMedGoogle Scholar
- Greinix HT, Reiter E, Keil F, Fischer G, Lechner K, Dieckmann K, Leitner G, Schulenburg A, Hoecker P, Haas OA, et al.: Leukemia-free survival and mortality in patients with refractory or relapsed acute leukemia given marrow transplants from sibling and unrelated donors. Bone Marrow Transplant. 1998, 21: 673-678. 10.1038/sj.bmt.1701152.View ArticlePubMedGoogle Scholar
- Wong R, Shahjahan M, Wang X, Thall PF, De Lima M, Khouri I, Gajewski J, Alamo J, Couriel D, Andersson BS, et al.: Prognostic factors for outcomes of patients with refractory or relapsed acute myelogenous leukemia or myelodysplastic syndromes undergoing allogeneic progenitor cell transplantation. Biol Blood Marrow Transplant. 2005, 11: 108-114. 10.1016/j.bbmt.2004.10.008.View ArticlePubMedGoogle Scholar
- Oyekunle AA, Kröger N, Zabelina T, Ayuk F, Schieder H, Renges H, Fehse N, Waschke O, Fehse B, Kabisch H, et al.: Allogeneic stem-cell transplantation in patients with refractory acute leukemia: a long-term follow-up. Bone Marrow Transplant. 2006, 37: 45-50.PubMedGoogle Scholar
- Brown RA, Wolff SN, Fay JW, Pineiro L, Collins RH, Lynch JP, Stevens D, Greer J, Herzig RH, Herzig GP: High-dose etoposide, cyclophosphamide, and total body irradiation with allogeneic bone marrow transplantation for patients with acute myeloid leukemia in untreated first relapse: a study by the North American Marrow Transplant Group. Blood. 1995, 85: 1391-1395.PubMedGoogle Scholar
- Brown RA, Wolff SN, Fay JW, Pineiro L, Collins RH, Lynch JP, Stevens D, Greer J, Herzig RH, Herzig GP: High-dose etoposide, cyclophosphamide and total body irradiation with allogeneic bone marrow transplantation for resistant acute myeloid leukemia: a study by the North American Marrow Transplant Group. Leuk Lymphoma. 1996, 22: 271-277. 10.3109/10428199609051758.View ArticlePubMedGoogle Scholar
- Duval M, Klein JP, He W, Cahn JY, Cairo M, Camitta BM, Kamble R, Copelan E, de Lima M, Gupta V, et al.: Hematopoietic stem-cell transplantation for acute leukemia in relapse or primary induction failure. J Clin Oncol. 2010, 28: 3730-3738. 10.1200/JCO.2010.28.8852.PubMed CentralView ArticlePubMedGoogle Scholar
- Slovak ML, Kopecky KJ, Cassileth PA, Harrington DH, Theil KS, Mohamed A, Paietta E, Willman CL, Head DR, Rowe JM, et al.: Karyotypic analysis predicts outcome of preremission and postremission therapy in adult acute myeloid leukemia: a Southwest Oncology Group/Eastern Cooperative Oncology Group Study. Blood. 2000, 96: 4075-4083.PubMedGoogle Scholar
- Moorman AV, Harrison CJ, Buck GA, Richards SM, Secker-Walker LM, Martineau M, Vance GH, Cherry AM, Higgins RR, Fielding AK, et al.: Karyotype is an independent prognostic factor in adult acute lymphoblastic leukemia (ALL): analysis of cytogenetic data from patients treated on the Medical Research Council (MRC) UKALLXII/Eastern Cooperative Oncology Group (ECOG) 2993 trial. Blood. 2007, 109: 3189-3197. 10.1182/blood-2006-10-051912.View ArticlePubMedGoogle Scholar
- Przepiorka D, Weisdorf D, Martin P, Klingemann HG, Beatty P, Hows J, Thomas ED: 1994 Consensus Conference on Acute GVHD Grading. Bone Marrow Transplant. 1995, 15: 825-828.PubMedGoogle Scholar
- Vogelsang GB: How I treat chronic graft-versus-host disease. Blood. 2001, 97: 1196-1201. 10.1182/blood.V97.5.1196.View ArticlePubMedGoogle Scholar
- Gooley TA, Leisenring W, Crowley J, Storer BE: Estimation of failure probabilities in the presence of competing risks: new representations of old estimators. Stat Med. 1999, 18: 695-706. 10.1002/(SICI)1097-0258(19990330)18:6<695::AID-SIM60>3.0.CO;2-O.View ArticlePubMedGoogle Scholar
- Storer BE: Statistical considerations in studies of late effects in HCT. Biol Blood Marrow Transplant. 2009, 15 (Suppl 1): 25-28. 10.1016/j.bbmt.2008.10.003.View ArticlePubMedGoogle Scholar
- Baron F, Maris MB, Sandmaier BM, Storer BE, Sorror M, Diaconescu R, Woolfrey AE, Chauncey TR, Flowers ME, Mielcarek M, et al.: Graft-versus-tumor effects after allogeneic hematopoietic cell transplantation with nonmyeloablative conditioning. J Clin Oncol. 2005, 23: 1993-2003. 10.1200/JCO.2005.08.136.View ArticlePubMedGoogle Scholar
- Schmid C, Schleuning M, Schwerdtfeger R, Hertenstein B, Mischak-Weissinger E, Bunjes D, Harsdorf SV, Scheid C, Holtick U, Greinix H: Long-term survival in refractory acute myeloid leukemia after sequential treatment with chemotherapy and reduced-intensity conditioning for allogeneic stem cell transplantation. Blood. 2006, 108: 1092-1099. 10.1182/blood-2005-10-4165.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.