Renal cancer-selective Englerin A induces multiple mechanisms of cell death and autophagy
© Williams et al.; licensee BioMed Central Ltd. 2013
Received: 27 June 2013
Accepted: 8 August 2013
Published: 20 August 2013
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© Williams et al.; licensee BioMed Central Ltd. 2013
Received: 27 June 2013
Accepted: 8 August 2013
Published: 20 August 2013
Renal cell carcinoma (RCC), the most common malignancy of the kidney, is refractory to standard therapy and has an incidence that continues to rise. Screening of plant extracts in search of new agents to treat RCC resulted in the discovery of englerin A (EA), a natural product exhibiting potent selective cytotoxicity against renal cancer cells. Despite the establishment of synthetic routes to the synthesis of EA, very little is known about its mechanism of action. The results of the current study demonstrate for the first time that EA induces apoptosis in A498 renal cancer cells in addition to necrosis. The induction of apoptosis by EA required at least 24 h and was caspase independent. In addition, EA induced increased levels of autophagic vesicles in A498 cells which could be inhibited by nonessential amino acids (NEAA), known inhibitors of autophagy. Interestingly, inhibition of autophagy by NEAA did not diminish cell death suggesting that autophagy is not a cell death mechanism and likely represents a cell survival mechanism which ultimately fails. Apart from cell death, our results demonstrated that cells treated with EA accumulated in the G2 phase of the cell cycle indicating a block in G2/M transition. Moreover, our results determined that EA inhibited the activation of both AKT and ERK, kinases which are activated in cancer and implicated in unrestricted cell proliferation and induction of autophagy. The phosphorylation status of the cellular energy sensor, AMPK, appeared unaffected by EA. The high renal cancer selectivity of EA combined with its ability to induce multiple mechanisms of cell death while inhibiting pathways driving cell proliferation, suggest that EA is a highly unique agent with great potential as a therapeutic lead for the treatment of RCC.
Renal cell carcinoma (RCC) is the most common type of malignant kidney tumor with an incidence that continues to rise. Between 1992 and 2005, the incidence of RCC rose by 1.8% and 2.1% among white men and white women, respectively . Although surgery can be curative for tumors confined to the kidney, about 25% of patients have metastatic disease at diagnosis, and another 20-40% develop metastases following surgery [2, 3]. The two-year survival rate for patients with metastatic disease is under 20% due to the poor response of these tumors to current treatments. Clear cell RCC (cc-RCC) which comprises 83% of RCC is one of the most radio- and chemo-resistant cancers and no curative treatment is available once metastases develop .
Investigations of the molecular biology of RCC have established that inactivating alterations in the Von Hippel Lindau (VHL) tumor suppressor gene are present in the majority (91%) of sporadic cc-RCC underscoring the central role of VHL in the regulation of growth and differentiation of renal epithelium [5–7]. The VHL gene product is involved in oxygen and energy sensing by regulating the activity of the hypoxia inducible factors (HIFs) . Inactivation of VHL results in HIF stabilization and the activation of transcription of over 60 hypoxia-responsive genes involved in oncogenesis and tumor progression including vascular endothelial growth factor (VEGF), the platelet-derived growth factor (PDGF), transforming growth factor alpha (TGF-α), epidermal growth factor (EGF), and glucose transporter-1 (GLUT-1) among others [9, 10]. Subsequent to the activation of HIF-inducible genes, a variety of downstream signaling pathways are activated of which the most studied are the RAF-MEK-ERK series of kinases and the phosphatidylinositol-3 kinase-protein kinase B-mammalian target of rapamycin (PI3K-AKT-mTOR) pathway . Based on the activation of these pathways in RCC, several targeted therapies have been developed including those against VEGF and PDGF receptors, and mTOR. However, despite the promise of approved targeted therapies for RCC, a complete response is rare and patients often become resistant/refractory to first line treatment [3, 12]. Thus, new agents with improved efficacy and decreased toxicity are needed as treatment options in first line or subsequent settings.
The need to identify new chemical motifs as potential drug leads has spurred the screening of plant extracts that are being used in traditional medicines [13, 14]. In particular, South Africa has a remarkable botanical diversity with over 30,000 flowering species, from which more than 3,000 are used for medicinal purposes throughout the country. Among them, plants of the genus Phyllanthus (Euphorbiaceae) are widely distributed in tropical forests throughout the world and have long been used in folk medicine to treat kidney and urinary tract infections . Based on this knowledge, Ratnayake et al.  at the NCI screened extracts of the Tanzanian plant Phyllanthus engleri and have reported the isolation of two novel bioactive sesquiterpenes, named englerin A (EA) and englerin B. Initial studies by the NCI demonstrated that EA possessed very potent growth inhibitory activity (GI50 = 10–87 nM) against most RCC with a selectivity that is approximately 1,000-fold higher compared to other cancers.
Although several synthetic routes toward the synthesis of EA have been established [16–21], other than EA’s selective toxicity to RCC, recently confirmed by us , very little is known about its biological actions and mechanism(s) of action. Only recently, one study reported that EA induced necrosis in RCC . The most recent report concluded that EA bound and activated protein kinase C-θ (PKCθ) to inhibit insulin signaling while, concurrently, activating HSF1, a known inducer of glucose dependence . This dual signaling, that promotes glucose addiction while inhibiting glucose uptake by the cells, was proposed to be the mechanism for the selective cytotoxicity of EA. Although the data presented is compelling, whether in fact this mechanism accounts for the cytotoxicity of EA is not yet clear. Based on its cytotoxicity profile against the NCI60 cell panel, EA is clearly a very unique agent and there is much to be learned about the actions of EA in RCC and the mechanisms and targets involved in these actions. In this study, using the highly EA-sensitive A498 human renal carcinoma cells as our model system, we report the results of a thorough and systematic investigation to uncover the mechanisms of growth inhibition and cell death induced by EA and reveal for the first time that EA induces multiple mechanisms of cell death as well as cell cycle arrest while inducing autophagy.
The A498 human kidney carcinoma cell line was purchased from ATCC and maintained in RPMI medium supplemented with 10% FBS and 100 units/ml penicillin/streptomycin (complete medium).
Englerin A was purchased from Cerilliant Corporation. (Round Rock, Texas). Rapamycin was purchased from Enzo Life Sciences (Farmingdale, NY) as part of the Cyto-ID® Autophagy Detection Kit. VP16 was purchased from Sigma Aldrich (St. Louis, MO). MEM 100X non-essential amino acids (NEAA) was purchased from Gibco Life Technologies (Grand Island, NY). Antibody against caspase-3 was a gift from Dr. Robert Naviaux and anti LC3B was purchased from Cell Signaling Technology (Danvers, MA). Antibody against β-actin (AC-15) was purchased from Sigma Aldrich (St. Louis, MO). Antibodies against phospho AMPK (Thr172) and phospho ERK (Thr202/Tyr204) as well as those for AMPK and ERK were generous gifts of Dr. R. Naviaux. The antibodies against AKT and phospho AKT (Ser473) were purchased from Cell Signaling Technology.
A498 cells were plated at 5,000 cells/well in a 96-well plate in complete medium. The following day, cells were treated with EA at 50 and 100 nM. Control cells received 0.1% DMSO. All conditions were performed in triplicate. Cells were then incubated with additions for 24 or 48 h before measuring viability using the PrestoBlue® (Invitrogen, CA) assay as described by manufacturer. This assay uses a resazurin-based solution that functions as a cell viability indicator by using the reducing power of living cells to quantitatively measure the proliferation of cells. Viability was determined by measuring fluorescence on a Synergy Mx plate reader (BioTek Instruments Inc., Winooski, VT) with excitation/emission at 560/590 nM.
Apoptosis was determined independently by two different methods. The Alexa Fluor® 488 annexin V/Dead Cell Apoptosis Kit (Life Technologies, Grand Island, NY) was used to measure externalized phosphatidyl serine and dead cells permeable to propidium iodide (PI). For these experiments, A498 cells were treated with 100 nM EA or with 0.1% DMSO (control) for 24 and 46 h. Cells were then trypsinized, washed with ice cold PBS, and stained with Alexa Fluor® 488 annexin V and PI as recommended by manufacturer. Cells were then analyzed by flow cytometry using a FACS Caliber flow cytometer (Beckton Dickinson, Franklin Lakes, NJ) and Flow Jo software (TreeStar Inc., Ashland, OR).
Apoptosis induced by EA in A498 cells was also determined by measuring cytoplasmic histone-associated-DNA-fragments using the Cell Death Detection ELISAPLUS kit (Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer’s instructions. In these experiments, A498 cells were plated at 5,000 cells/well (96-well plate) in complete RPMI medium. The following day, cells were treated with 100 nM EA or with 0.1% DMSO, and incubated at 37°C for 18, 24, and 45 h before apoptosis was measured.
Multiple caspases were analyzed using the FLICA reagent (FAM Caspase Activity kit, Imgenex, San Diego, CA) which only binds active caspases. In these experiments, A498 cells were plated at 0.5 × 106 cells/T-25 flask in complete RPMI. After cells were allowed to attach overnight, cells were treated with 100 nM EA or 0.1% DMSO for 43 h, or with 200 μM etoposide for 24 h. Cells were then harvested and stained with the FLICA reagent according to manufacturer’s recommendations and fluorescence was measured with excitation at 490 nm and emission at 520 nm. Caspase-9 activity was measured after treatment of cells with and without 100 nM EA as above followed by trypsinization and cell lysis. Caspase-9 activity was then determined using the Caspase-9 Colorimetric Assay kit (BioVision, Mountain View, CA) according to protocol provided by manufacturer. Absorbance was read at 400 nm. The levels of active caspase-3 were determined by Western blot analysis as described below.
Autophagy was determined by three different methods including flow cytometry, fluorescence microscopy and western blot analysis. For flow cytometry experiments, A498 cells were plated in T-75 flasks at 1.25 × 106/flask in complete RPMI. After the cells were allowed to attach overnight, cell were treated with 200 nM EA or 0.1% DMSO (control) for 46 h and with 500 nM rapamycin for 20 h. Autophagy was measured by staining autolysosomes and earlier autophagic compartments with the fluorescent probe Cyto-ID® Green (Enzo Life Sciences, Farmingdale, NY) as recommended by manufacturer. Samples were then analyzed in the green (FL1) channel of the FACS Caliber flow cytometer.
For fluorescence microscopy, A498 cells were plated in complete RPMI on coverslips placed in a 60 mm dish at 1.5 (control cells) to 3.0 × 105 (treated cells) cells/dish. After the cells were allowed to attach overnight, cell were treated with 200 nM EA or 0.1% DMSO (control) for 45 h. Cells were then stained with Hoechst nuclear stain and Cyto-ID® Green detection reagent using the Cyto-ID® Autophagy Detection Kit according to recommendations. Cells were fixed with 4% formaldehyde for 20 min at room temp followed by three washes with 1X assay buffer. Cover slips were then placed on slides with mounting media. Stained cells were analyzed by fluorescence microscopy (Olympus BX51 microscope that has been equipped with the fluorescence illuminator BX-URA2) using an Omega Optical XF100-2 filter for green bandpass with a 475 nm exciter to image autophagic cells.
A498 cells were plated at 1–2 × 106 cells/ T-75 flask in complete RPMI. After cells were allowed to adhere overnight, cells were treated with 100, 200 nM EA or with 0.1% DMSO for 48 h before harvesting. Cells were trypsinized, collected, and resuspended in ice- cold PBS. Cells were lysed in RIPA buffer (50 mM Tris–HCl pH 8.0, 1% Triton X-100, 150 mM NaCl, 1mM EDTA, 0.5% Deoxycholate, 0.1% Sodium Dodecyl Sulfate, 1mM Sodium Fluoride, 1 mM Sodium Pyrophosphate) in the presence of PMSF and protease inhibitor cocktail. Lysates were clarified by centrifugation for 15 min at 10,000×g, 4°C. To the clarified lysate, 4 × NuPAGE LDS sample buffer (Life Technologies) and 0.05 M dithiothreitol were added and samples were heated for 10 min at 80°C. Proteins were separated by SDS-PAGE on a 10% Bis-Tris NuPAGE Gel (Life Technologies) and then transferred to PVDF membranes (Bio-Rad). The PVDF membranes were blocked with 5% Bovine Serum Albumin (Sigma) in TBS with 0.05% Tween-20 and probed with antibodies against caspase-3, (diluted 1:1000), LC3B (diluted 1:1000), and B-actin (diluted 1:50,000). Antibodies against AMPK, AKT, ERK and against the corresponding phospho proteins were each diluted 1:1000 except for phospho AKT which was diluted at 1:500. An HRP-conjugated anti-mouse antibody (diluted 1:1000) or HRP-conjugated anti-rabbit antibody (diluted 1:1000;) was used as a secondary detection probe. Bands were visualized using ECL enhanced chemiluminescent substrate (Pierce) and exposed to HyBlot CL film (Denville Scientific). The film was developed with a Kodak film developer.
A498 cells were plated at 2 × 105 (control) or at 4 × 105 (EA treated) cells/flask into T-25 flasks in complete RPMI. After cells were allowed to attach overnight, cells were treated with 200 nM EA or with 0.1% DMSO for 45 h. The cells were then trypsinized, washed with ice-cold PBS, fixed with ice-cold 70% ethanol at a 1:10 ratio of cell suspension to 70% ethanol, and stored at -20ºC overnight. Cells were washed twice with PBS and then stained with staining solution containing Triton x-100 (0.1% v/v), DNase free RNase (200 μg/ml), PI (30 μg/ml) in PBS for 15 min at 37ºC. PI content of cells was measured using a FACS Calibur flow cytometer and cell cycle distribution was determined using FlowJo analysis software.
Having demonstrated that EA induces autophagy in A498 cells, the question that arises is whether autophagy is a defense mechanism or a cell death mechanism. To answer this question, both cell viability and levels of apoptosis were determined in independent experiments in which A498 cells were treated with and without NEAA (1X) in the presence and absence of 150 nM EA, or with 200 μM VP16 for 46 h. As shown in Figure 4B, the viability of cells treated with EA were similar to that receiving EA plus NEAA as determined by the PrestoBlue® assay. NEAA, alone, had no effect on the cells when compared to control cells receiving vehicle (0.1% DMSO), whereas, cells treated with VP16 lost viability as expected. These results indicated that inhibition of autophagy did not diminish cell death induced by EA. We then examined the levels of apoptosis in A498 cells treated in the same manner as in the viability experiments. The results of these experiments demonstrated that the levels of apoptosis were similar in cells treated with EA compared to those treated with EA plus NEAA indicating that inhibiting autophagy does not affect the level of apoptosis induced by EA (Figure 4C). It is noteworthy that the level of apoptosis induced by EA appears to be much less than that induced by VP16 (Figure 4B) even though the agents reduce cell viability to similar levels (Figure 4A). Taken together, our results suggest that EA-induced autophagy does not appear to be a cell death mechanism, and is likely a defense mechanism that ultimately fails and cells die by a caspase-independent apoptotic cell death and by necrosis (Figures 1B and C).
In summary, our results demonstrate that EA induces cell death in A498 cells by caspase-independent apoptosis and necrosis while inducing autophagy. Inhibition of autophagy does not diminish cell death by EA suggesting that autophagy is not a cell death mechanism and is likely a survival mechanism which ultimately fails. In addition to inducing cell death, EA arrests cells in G2 phase of the cell cycle blocking the G2/M transition. Taken together, our results indicate that cell death by EA occurs by multiple mechanisms which are likely cell context dependent. Because EA can elicit cell death by multiple mechanisms and can inhibit multiple pathways that drive cell proliferation, it has the potential to be an effective chemotherapeutic agent that can bypass chemo-resistance, making it ideal for the treatment of metastatic RCC.
Metastatic RCC is one of the most chemo-resistant cancers for which no curative treatment is available. Hallmarks of this cancer include a highly hypoxic and glycolytic nature and an increased dependency on glucose, all characteristics associated with VHL loss and HIF stabilization which play a central role in the pathogenesis of RCC. However, the limited success of therapeutics targeting the VHL/HIF axis suggests that other molecular alterations also play an important role in the development of RCC. Since pVHL loss and HIFα stabilization are the earliest detectable molecular events in VHL-associated renal tumorigenesis, it is believed that these initial changes trigger other events, both HIF-dependent and independent, resulting in progression to RCC. For example, increased hepatocyte growth factor signaling through c-MET, increased susceptibility to TGF-α/EGF signaling, as well as modifications in extracellular matrix turnover and remodeling are implicated in the pathogenesis of RCC . Clearly, RCC is a complex disease resulting from numerous alterations of genes and pathways that work in concert, indicating that pursuing a single target or pathway will not yield chemotherapeutics with significant efficacy. The best chance for achieving therapeutic efficacy in a disease such as RCC should involve the use of agents that target the multiple pathways which contribute fundamentally to this disease.
Natural products are well known to affect multiple targets and thus have excellent potential as chemotherapeutic agents. The relatively recently identified natural product, englerin (EA), is very unique due to its high selectivity against RCC that is 1000-fold higher than any other cell type . Our results demonstrate that EA induces apoptosis and autophagy in addition to necrosis in A498 RCC cells at nanomolar concentrations. This finding is in contrast to a recent report stating that EA induced necrosis but not apoptosis or autophagy . In this previous study, however, autophagy was most likely inhibited by the supplementation of culture medium with non-essential amino acids (NEAA), a known inhibitor of autophagy , and was thus not observed. Our results confirmed that autophagy induced by EA could be inhibited by NEAA. We further showed that inhibition of autophagy by NEAA did not diminish cell death. This finding is supported by the previous study which showed that RCC cells died under conditions which inhibited autophagy with a sensitivity to EA similar to that observed by us and others [16, 21]. For instance, in viability assays in the study by Sulzmaier et al. , EA was found to have an EC50 of 53 nM in the presence of NEAA. In the absence of NEAA, the estimated EC50 of EA in A498 cells in our viability assay was 63 nM (Figure 1 and data not shown). Furthermore, the NCI reported LC50 for EA in A498 cells, under conditions not inhibiting autophagy, was 79 nM . Though the NCI determined LC50 is a somewhat different measure than the EC50, determined by us and Sulzmaier et al. , in addition to the assays being different, the fact that these values are not very different regardless of whether autophagy is inhibited, indicates that autophagy does not appear to have much of an effect on cell death. Though autophagy can play a pro-death role when prolonged or in certain developmental conditions , in most circumstances, autophagic generation of nutrients prevents or delays cell death , thus acting as a survival mechanism. It is, in fact, fairly common for cancer cells experiencing stress of different origin to activate autophagy in an attempt to alleviate stress and survive . It is for this reason, that the autophagic machinery has become a therapeutic target. Inhibiting autophagy in tumor cells exposed to cytotoxic agents often results in increased apoptotic cell death . However, we have not observed this in the context of EA-induced apoptosis as the levels of apoptosis were not altered by the inhibition of autophagy by NEAA (Figure 4). It is not entirely clear what role EA-induced autophagy plays in in A498 cells, but it does not appear to represent a cell death mechanism in this context, and most likely is a survival mechanism that ultimately fails.
Although EA induced apoptosis in A498 RCC cells, it did not appear to be a strong inducer of apoptosis as compared to other agents such as VP16 and camptothecin (Figure 4 and data not shown). Interestingly, the report by Sulzmaier et al.  concluded that EA did not induce apoptosis in these cells. However, by analyzing not only external exposure of phosphatidyl serine, but also by examining histone-associated DNA fragments, we found that EA did induce some level of apoptosis in A498 cells. The induction of apoptosis by EA was independent of caspase activation suggesting the involvement of non caspase proteases such as cathepsins and calpains . It is likely that the induction of apoptosis by EA is cell context dependent and, thus, may not be induced in all RCC cells, especially, considering that certain cells may have an apoptotic block. In such a case, EA may induce other mechanisms of cell death such as necrosis as observed by Sulzmaier et al. . Our results indicated that EA also induced necrosis as determined by PI staining (Figure 1C). Taken together, our results indicate that EA can induce cell death by multiple mechanisms and that the predominant mechanism will depend on cell context.
In addition to inducing cell death, EA also induced a block in the G2/M transition of the cell cycle in A498 cells. This indicated that EA may likely regulate cell cycle regulatory genes and affect pathways associated with cell proliferation. In fact, our results indicated that EA inhibited activation of both AKT and ERK, members of two pathways commonly activated in cancer, often together , and which are associated with unrestricted cellular proliferation and decreased sensitivity to apoptosis-inducing agents . It is known that inhibition of either pathway alone has a negligible effect on tumor growth and survival suggesting that these pathways share downstream targets . The fact that EA can inhibit activation of both pathways suggests that it would be an effective agent in inhibiting tumor growth. This possibility is supported by the findings of a very recent study of EA in athymic mice bearing 786–0 (renal) tumor xenografts . The results of this study demonstrated that EA markedly inhibited tumor growth over a two week period when administered daily at 5 mg/kg intraperitoneally. This study further showed that tumors excised from the EA-treated mice revealed increased inhibitory phosphorylation of the insulin receptor substrate 1 (IRS1) and decreased activity of the PI3/AKT pathway, in line with our in vitro results in A498 cells. Based on their in vitro results, the authors of this study concluded that EA bound and activated PKCθ to inhibit insulin signaling while, concurrently, activating HSF1, a known inducer of glucose dependence, thus, starving cells of glucose while promoting glucose addiction. However, because the in vitro binding studies with EA and PKCθ were indirect without any binding kinetic analyses, it is unclear if PKCθ is a primary target of EA. Furthermore, the experiments demonstrating inhibition of glucose uptake by EA were performed using EA at 10 μM, a concentration of EA approximately 200-fold higher than its IC50. It is well established that when cells are starved, the energy sensor, AMP-activated protein kinase, becomes activated by phosphorylation resulting in the induction of autophagy. If EA inhibits glucose uptake, it would be expected to result in a higher ADP/ATP and AMP/ATP ratio and consequent activation of AMPK. Our results, however, did not reveal activation of AMPK by EA at a concentration of 100 nM, a concentration that is highly cytotoxic to A498 cells. Hence, it is possible that the effects of EA on glucose uptake may occur at micro molar concentrations that are much higher than required for cell death (nanomolar) and could represent off-target effects. Moreover, as a natural product, EA would be expected to have multiple targets and most likely has targets in addition to PKCθ. Such targets may include those associated with the ER stress since it is well established that ER stress results in the induction of cell death and autophagy . An example of agent that induces autophagy and cell death by inducing ER stress in RCC includes STF-62247 which targets VHL-deficient RCC . EA may target proteins within the Golgi complex analogous to carminomycin I, a natural product with selective toxicity to VHL-deficient CC-RCC .
In conclusion, EA induces cell death via multiple mechanisms and likely has multiple cellular targets. The identification of these targets and pathways affected by this unique agent will be invaluable in understanding the high RCC- selectivity of EA and allow development of highly effective chemotherapeutics for the treatment of metastatic RCC, a highly treatment resistant cancer.
Renal cell carcinoma
Heat shock factor 1
Von Hippel Lindau.
We gratefully acknowledge Dr. Stoyan Dimitrov for his assistance with the flow cytometry studies.
This work was supported by a fund from Academia Sinica (A. L. Yu) and, in part, by an NIH grant (CA 133002) awarded to Emmanuel Theodorakis.
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