Fig. 3

PDO response to HIPEC simulation. A Schematic representation of the protocol used to perform the dose–response curves experiments. B Description the five HIPEC schemes tested. C Dose–response curves of CRCPM-derived PDOs illustrating the variation in sensitivity to MMC (scheme 1), MMC + CDDP (scheme 2), DOX + CDDP (scheme 3), L-OHP_low-dose (scheme 4) and L-OHP_high-dose. (scheme 5). D Normalized Log of IC₅₀ mean values heat-map for standard HIPEC treatments (yellow: no response; blue: good response). HIPEC Hyperthermic IntraPEritoneal Chemotherapy, CRCPM Colorectal Cancer Peritoneal Metastasis, PDO Patient-derived Organoid, MMC Mitomycin-C, CDDP Cisplatin, DOX Doxorubicin, L-OHP Oxaliplatin, IC₅₀ Half maximal inhibitory concentration