Figure 1

Effect of HIPK2 on VEGF and β-catenin levels. (A) MCF7 cells were transfected with HIPK2 or the K221R kinase defective mutant and 24 and 48 h after transfection VEGF and β-catenin (β-cat) mRNA levels were evaluated by reverse transcriptase-PCR (RT-PCR) analysis. GAPDH was used as loading control. One representative experiment from three independent experiments was shown. (B) Cells were transfected as in (A) and 36 h after transfection cell extracts were subjected to SDS-PAGE and immunoblotting was performed with anti-β-catenin and anti-cyclin D1 (cycl D1) antibodies. Anti-tubulin was used as protein loading control. One representative experiment from three independent experiments was shown.