Dark Aberrant Crypt Foci with activated Wnt pathway are related to tumorigenesis in the colon of AOM-treated rat

Table 3 Immunohistochemical analysis for the expression of β-catenin, mmp-7 in classic ACF, dark ACF and tumor

	β-catenin				mmp-7
	No. of normal samples/total No. of samples	No. of samples with reduced expression in membrane/total No. of samples	No. of samples with overexpression in cytoplasm/total No. of samples	No. of samples with accumulated expression in nucleus/total No. of samples	No. of samples with mmp-7 expression/total No. of samples	No. of samples without mmp-7 expression/total No. of samples
Classic ACF	157/165	4/165	4/165	0/165	13/165	152/165
Dark ACF	0/22	6/22	8/22	8/22	18/22	4/22
Tumor	0/10	0/10	3/10	7/10	10/10	0/10

Note: The rate of ectopic expression of β-catenin in classic ACF is significantly lower than that in tumors (P = 0.0023). The rate of mmp-7 expression in classic ACF is significantly lower than that in tumors (P = 0.000). The rate of β-catenin ectopic expression and positive expression of mmp-7 in dark ACF is not significantly different with those in tumor (P = 0.143 and P = 0.283, respectively). The rate of ectopic expression of β-catenin in dark ACF is significantly higher than that in classic ACF (P = 0.000). The rate of mmp-7 expression in dark ACF is significantly higher than that in classic ACF (P = 0.000).

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