Figure 3
HPV-18E6 promotes multiple sites phosphorylation of p53 along with up-regulation of ATM and Chk2. The phosphorylated responses appear obviously at three sites: Ser15, Ser20, and Ser392 of p53 along with the up-regulation of ATM and Chk2 at 24 h in GFP-18E6 expressing 293T and MCF-7 cells. IgG is an irrelative antibody used as the negative control. Data are normalized to β-actin and representative of three independent western blot analyses.