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Figure 4 | Journal of Experimental & Clinical Cancer Research

Figure 4

From: In GFP with high risk HPV-18E6 fusion protein expressed 293T and MCF-7 cells, the endogenous wild-type p53 could be transiently phosphorylated at multiple sites

Figure 4

Localization and expression level of phosphorylated p53 proteins. (A) In 293T and MCF-7 cells, the phosphorylated p53s are located in nuclei together with GFP-18E6. Green fluorescence indicates the protein of GFP, and GFP-18E6 expressed by the transfected cells, Red fluorescence indicates phosphorylated p53 proteins, which are labelled with phosphorylated anti-p53 antibodies plus anti-rabbit-Cy3 secondary antibody. The photographs are examined at 400× magnification by confocal microscopy. Scale bar = 8 μm. The results shown are representative of three independent experiments. (B) Level of phosphorylated p53 in the context of GFP-18E6 from 12 h to 72 h. The data of phosphorylated p53s level are examined by fluorescence intensity. 100 cells are examined for each phosphorylated site of p53 from 20× random fields.

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