Figure 1
From: Sp1 is involved in H2O2-induced PUMA gene expression and apoptosis in colorectal cancer cells
H 2 O 2 induced PUMA expression and apoptosis in a time-and dose-dependent manner in colorectal cancer cells. (A) LoVo Cells were treated with 0.64 mM H2O2 at different time point and PUMA expression was assessed by Western blotting analysis. (B) Western blotting analysis of a dose-range of a 15 minute H2O2 exposure of LoVo cells. Data was presented as the percentage of the basal level of PUMA expression (control = 100%) in the absence of H2O2 and expressed as mean ± SD of three independent experiments. (C, D) LoVo Cells were treated with a time- and dose- range of H2O2 as indicated in Figure 1C, D, apoptosis was assessed by Hoechst dye 33258. Data was presented as the number of apoptosis per 200 cells in three different areas. Statistical significance as compared to control cells (*p < 0.05, #p > 0.05).