Figure 6

Terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling staining of tumor tissues. Sections after treatment were stained by TUNEL analysis to detect apoptotic cells. Percent apoptosis was determined by counting the number of apoptotic cells and dividing by the total number of cells in the field (5 high power fields/slide). (A) Representative Field from each group. (a) NS group, (b) PORF-9 null plasmid group, (c) Msurvivin T34A plasmid group. (B) Percent apoptosis in each group. Values were expressed as means ± SE. Msurvivin T34A plasmid:lipo complexes increased the percent apoptosis in breast tumor sections relative to either normal saline or PORF-9 null plasmid:lipo complexes. (p < 0.05, respectively).