CD147 expression in SMMC-7721 cells transfected with negative control siRNA or siRNA-CD147. After SMMC-7721 being transfected with negative control siRNA or si-CD147 for 48 h, CD147 expression was detected using flow cytometry (A, B) and western blot assay(C, D). GAPDH as a loading control was shown in the bottom panel. The PVDF membranes were washed and the bands were scanned by Odyssey Infrared Imaging System (C). The graph (D) compares scanning signal intensity of CD147 by ImageJ software.