Figure 8

Effects of SAPK/JNK inhibitor SP600125 on gelatinase production and expression. Total cellular protein from SMMC-7721 cells treated with SP600125(100 μM) for 1 h was analyzed by gelatin zymography and western blot assay. The results showed that MMP-2 production (A) and expression (C), and MMP-9 expression (E) in SMMC-7721 cells treated with SP600125 was significantly inhibited. The graph (B, D, F) compares scanning signal intensity of MMP-2 and MMP-9 production and expression by ImageJ software.