Figure 1

BSM induced, methylation-specific sequence differences in the DPYD promoter region. Sequence chromatograms of clone inserts originating from methylated and unmethlyated DNA, obtained in the control experiment using enzymatically methylated DNA. Unmethylated cytosines at CpG sites (indicated with black arrows) are converted to thymines, resulting in a sequence change. The two regulatory elements are shown; numbers indicate the sequence position relative to the transcription start site.