PI3-kinase inhibitor enhances the growth inhibitory effects of cisplatin. Cell proliferation was measured by MTS assay. The combined administration of 10 μmol/L LY294002 and 5 μmol/L cisplatin inhibited cell growth in AsPC-1 (A) and PANC-1 (B) significantly. Values represent the means (± SD) of n = 5. *, + and # compared with control, cisplatin treatment and LY294002 treatment group, respectively (P < 0.05).