Augmentation therapy of LY294002 enhanced the apoptotic pathway in pancreatic cancer. Cytoplasmic histone-associated DNA fragments were determined by ELISA-based quantification. The pathway was increased significantly 48 hours after LY294002 administration, but apoptosis was not prominent following cisplatin treatment in AsPC-1 cell lines (A). The addition of 10 μmol/L LY294002 increased apoptosis in the presence of cisplatin synergistically. PANC-1 cell lines showed the same results (B). Values represent the means (± SD) of n = 5. *, + and # compared with control, cisplatin treatment and LY294002 treatment group, respectively (P < 0.05).