Figure 3

Effects of TRD, TRAIL and combination of both agents on viability, apoptosis and necrosis in HT1080 cells measured by FACS-analysis: Cells were incubated with 250 μmol/l TRD and 50 ng/ml TRAIL alone and in combination as well as with Povidon 5% + H₂O (control) for 2 h, 6 h, 12 h, and 24 h. The percentages of viable, apoptotic and necrotic cells were determined by FACS-analysis for Annexin V-FITC and Propidiumiodide. Values are means ± SD of 3 independent experiments with consecutive passages (*** p ≤ 0.001, ** p ≤ 0.005; one way ANOVA). The indicators of significance refer to the difference between the TRD 250 μmol/l + TRAIL 50 ng/ml and the control series. The differences in the values compared to the single dose experiments are caused by experimental variability. The scales of the y-axis were adjusted to the different values for clarity and therefore vary.