Figure 3
From: TRAIL and Taurolidine induce apoptosis and decrease proliferation in human fibrosarcoma

Effects of TRD, TRAIL and combination of both agents on viability, apoptosis and necrosis in HT1080 cells measured by FACS-analysis: Cells were incubated with 250 μmol/l TRD and 50 ng/ml TRAIL alone and in combination as well as with Povidon 5% + H2O (control) for 2 h, 6 h, 12 h, and 24 h. The percentages of viable, apoptotic and necrotic cells were determined by FACS-analysis for Annexin V-FITC and Propidiumiodide. Values are means ± SD of 3 independent experiments with consecutive passages (*** p ≤ 0.001, ** p ≤ 0.005; one way ANOVA). The indicators of significance refer to the difference between the TRD 250 μmol/l + TRAIL 50 ng/ml and the control series. The differences in the values compared to the single dose experiments are caused by experimental variability. The scales of the y-axis were adjusted to the different values for clarity and therefore vary.