Figure 10

Cilengitide and temozolomide exert synergistic anti-proliferative and pro-apoptotic effects in glioma cells. Proliferation. Cell lines G44 (A) and G28 (B) were treated with medium containing 4% FCS and DMSO, cilengitide (5 μg/ml) or/and temozolomide (5 μg/ml). Cells were trypsinized and counted after 24 and 48 hours incubation at 37°C. Apoptosis. Floating and attached G44 (C) and G28 (D) cells were collected 48 hours after treatment with cilengitide and or temozolomide at the indicated concentrations. Apoptosis was assessed by flow cytometry after annexin V and propidium iodide staining. Results from 1 of 4 experiments with similar results are shown.