Figure 6

Cilengitide inhibits phosphorylation of FAK, Src and Akt in glioma cells. G28 cells were treated with 50 μg/ml cilengitide for 30, 60 and 120 minutes at 37°C. Cell lysates containing similar amounts of protein were separated by SDS-PAGE, transferred to a nitrocellulose membrane and probed with specific antibodies for detection of β-actin and phosphorylated FAK and Akt (A). pFAK and pAkt were quantified by densitometry (OD/mm²) (B). G28 cells were treated with cilengitide at the concentrations indicated for 1 hour at 37°C. Cell lysates were separated by SDS-PAGE and transferred to a nitrocellulose membrane to detect β-actin and phosphorylated Src and Akt (C). pSrc and pAkt were then quantified by densitometry (OD/mm² (D).