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Table 1 Microsatellite primer for MSI assay

From: Microsatellite instability at a tetranucleotide repeat in type I endometrial carcinoma

Primer

Primer sequence

AT(°)

PCRcycle

Repeat type

BAT25

Forward : 5'-TCGCCTCCA AGAATGTAA GT-3'

55

28

Mono

 

Reverse: 5'-TCTGGATTTTAACTATGGCTC-3'

   

BAT26

Forward : 5'-TGACTACTTTTGACTTCAGCC-3'

55

30

 
 

Reverse: 5'-GTTTCTAACCATTCAACATTTTTATCCC-3'

   

BAT40

Forward : 5'-GTAGAGCAAGACCACCTTG-3'

55

30

 
 

Reverse: 5'-GTTTCTACAACCCTGCTTTTGTTCCT-3'

   

D2S123

Forward : 5'-AAACAGGATGCCTGCCTTTA-3'

55

35

Di

 

Reverse: 5'-GGACTTTCCACCTATGGGAC-3'

   

D17S250

Forward : 5'-GGAAGAATCAAATAGACAAT-3'

55

35

 
 

Reverse: 5'-GCTGGCCATATATATATTTAAACC-3'

   

D8S554

Forward : 5'-TTTCCAGACAGGGCCTA-3'

58

35

 
 

Reverse: 5'-AATGCACAGGACATAATTT-3'

   

MYCL1

Forward : 5'-TGGCGAGACTCCATCAAAG-3'

58

35

Tetra

 

Reverse: 5'-CCTTTTAAGCTGCAACAATTTTC-3'

   

D19S394

Forward : 5'-AGACTACAGYGAGCTGTGG-3'

58

35

 
 

Reverse: 5'-GTGTTCCTAACTACCAGGC-3'

   

D9S242

Forward : 5'-GTGAGAGTTCCTTCTGGC-3'

56

35

 
 

Reverse: 5'-ACTCCAGTACAAGACTCTG-3'

   

UT5320

Forward : 5'-ACCGACAGACTCTTGCCTC-3'

58

35

 
 

Reverse: 5'-TTGAGATGACCCTGAGACTG-3'

   

D8S321

Forward : 5'-GATGAAAGAATGATAGATTACAG-3'

58

35

 
 

Reverse: 5'-ATTCTTCTCATGCCATATCTGC-3'

   

L17686

Forward : 5'-GCACCAATGCTCCAGAAATG-3'

63

35

 
 

Reverse: 5'-TCATGGTGCCATGATAGGAG-3'

   
  1. AT : Annealing temperature.
  2. Mono : Mononucleotide repeat.
  3. Di : Dinucleotide repeat.
  4. Tetra : Tetranucleotide repeat
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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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