Figure 4

Risedronate inhibited the gelatinolytic activities of MMP-2 and MMP-9. (A) Conditioned media harvested from SaOS-2 and U2OS cells treated for 48 h with the indicated concentrations of risedronate were analyzed by gelatin zymography. The white bands represent MMP-mediated gelatin digestion. The image is representative of three independent experiments. MMPs activities (expressed as percentages of controls) are shown in B (n = 3). Numbers in boxes represent the concentration of risedronate (in μM) added to cells. Bars represent the MMPs activities (expressed as percentages of controls) of each band ± standard deviation.