Figure 3
Imprinting analysis of H19 in gastric cancer. H19 heterozygosity showed 655 bp DNA PCR product yielded bands of 487 and 168 bp by RsaI digestion (G1, G2). Normal tissues (N1, N2) showed only one allele expression indicating maintenance of normal imprinting (displayed 407 and 168 bp, 575 bp respectively by RsaI digestion RT-PCR products). T1, T2 displayed both three bands (575, 407 and 168 bp respectively) in tumor tissues indicating loss of imprinting in contrast to their matching normal tissues (N1, N2). M, marker DL2000. Nc1, Nc2 represented RT-PCR without reverse transcriptase.