A. Chromatin Immuno-Precipitation analysis of Sp1 binding to the ADAM17 promoter. Lanes 1-3 are negative controls for immuno-precipitation. Lanes 4-6 are the negative controls for the DNA optimization. The band in lane 7 indicates Sp1 binding within the ADAM17 promoter within 1-97 bp sequence. Lanes 8 and 9 indicate no Sp1 binding for the 356-455 and 781-879 regions of the ADAM17 promoter, respectively. B. The promoter sequence of ADAM17 from base pair one up to base pair 97. The arrows indicate the predicted human Sp1 binding site (3-9 bp).