Figure 4From: Transcription factor Sp1 induces ADAM17 and contributes to tumor cell invasiveness under hypoxiaEffect of Sp1 siRNA transfection upon invasiveness of U87 tumor cells under normoxic and hypoxic conditions. A. U87 cell invasion at 4 × objectives: N: normoxic incubation, H: hypoxic incubation, U87: control cells, Sp1-DR: U87 cells expressing Sp1 siRNA. 1. U87 control cells with transfected empty vector under normoxic conditions. 2. U87 control cells subjected to hypoxic incubation. 3. Sp1-deficient U87 cells under normoxic conditions. 4. Sp1-deficient U87 cells under hypoxic conditions. B. Invasive cell number compared to normoxic control. *P < 0.05 compared to normoxic control. #P < 0.05 compared to hypoxic control.Back to article page