Figure 4

Antibody to Tpit/E cells generated in the vaccinated mouse. Immunofluorescence (a, b) and corresponding phase contrast microscopic images (c, d) of cultured cells stained with a representative hybridoma-conditioned media, (a, c): B16/F10 cells, (b, d): Tpit/E cells. Hybridomas were produced from spleen cells of a Tpit/E-vaccinated mouse. The rim of Tpit/E cells was strongly stained, while B16/F10 was not, suggesting that the hybridoma secreted specific IgG reactive to the surface of Tpit/E cells.