Figure 3

PLK-1 knock-down by siRNA transfection modulated apoptosis in HeLa cells. A, Control; B, Cells transfected with PLK-1; C, Cells transfected with PLK-1 siRNA; D, Cells transfected with PLK-1 siRNA and treated with cisplatin (4 μg/ml) (original magnification, 200×); Enhanced apoptosis was demonstrated in B, C and D by typical nuclear condensation after siRNA transfection, as determined by Hoechst staining. Three independent experiments were performed. Representative fluorescent images are presented.