Figure 1From: Generation of fusion protein EGFRvIII-HBcAg and its anti-tumor effect in vivoMap of Pep3-HBcAg/pET-28a(+) prokaryotic expression plasmid. The three DNA fragments were ligated and subcloned into plasmid pGEMEX-1. Then fusion gene Pep3-HBcAg was digested with restriction enzymes Eco RI and Sal I and ligated into the equivalent sites of the pET-28a(+) vector, yielding His-tagged Pep3-HBcAg/pET-28a(+).Back to article page