Table 1 Sequence of primers used for PCR amplification
From: Generation of fusion protein EGFRvIII-HBcAg and its anti-tumor effect in vivo
Gene | Primers sequence | Restriction site |
|---|---|---|
Pep-3 | Sense: 5'-CCTCGAGCTGGAGGAGAAGAAAGGTAATTATGTG-3' Antisense: 5'-CGGATCCGTGATCTGTCACCACATAATTACC-3' | Xho l Bam HI |
HBcAg1-71 | Sense:5'-GGAATTCATGATTACGCCAAGCTTGGCTGCAGAGTTCCATATG-3' Antisense: 5'-CCCTCGAGTCCACCCCAGGTAGCTAGAGTC-3' | EcoR I Xho l |
HBcAg89-144 | Sense: 5'-CGGATCCGGTGGAGTCAACAACACTAATATGGGC-3' Antisense: 5'-CCAAGCCTTCCGCGGAAGTGTTGATAGGAT-3 | Bam HI Hind III |