Figure 2

Indirect immunoperoxidase staining of the NeuGc-GM3 ganglioside with 10 μg/ml of 14F7 monoclonal antibody on formalin-fixed B16 (A, B and E) and F3II (C, D and F) monolayers, cultured in the presence (B and D) or absence (A and C) of 10% FBS or incubated with 250 μg/ml mucin in FBS-free medium for 24 h (E and F). Original magnification 1000×.