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Figure 3 | Journal of Experimental & Clinical Cancer Research

Figure 3

From: Exogenous incorporation of neugc-rich mucin augments n-glycolyl sialic acid content and promotes malignant phenotype in mouse tumor cell lines

Figure 3

Detection of NeuGc-GM3 in cell membrane fraction by slot blot assay in B16 (A and B) and F3II (C and D) cells. A and C, tumor cells were preincubated with different concentrations of NeuGc-rich BSM and processed 24 h later. B and D, tumor cells were preincubated with 250 μg/ml of NeuGc-rich BSM and further processed 24, 48 or 72 h after preincubation. In all cases, densitometric analysis was normalized to the respective control. Means ± SEM of at least 3 determinations are shown. *p < 0.05, **p < 0.01 (ANOVA contrasted with Dunnet test).

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