Figure 4

A, adhesion assay. B16 or F3II cells were incubated for 1 h in medium with 2% FBS, either with (filled bars) or without (empty bars) 50 μg/ml of purified NeuGc. Data represent mean ± SEM (n = 6). *p < 0.05, **p < 0.01 (t test). B, proliferation assay. B16 (black square) or F3II (black diamond) cells were grown for 72 h in medium supplemented with 1, 5 or 10% FBS, either with or without 100 μg/ml of purified NeuGc. Dashed line refers to proliferation in control monolayers without addition of NeuGc. Data represent mean of 6 determinations; in all cases SEM was less than 5%. ***p < 0.001 versus the respective control (ANOVA contrasted with Tukey-Kramer multiple comparisons test).