Figure 1

Schematic presentation and purity ofhDM-αH-C6 MH3B1. (A), Schematic diagram of hDM-αH-C6 MH3B1. Each monomer of hDM is shown as filled oval. The αH linker, fused to the C-terminus of hDM is shown in black and C6 MH3B1 is shown as hexagons. (B), SDS-PAGE analysis under non-reducing and reducing conditions of purified hDM-αH-C6.5 MH3B1 visualized by Coomassie Blue staining. Lanes 1, 4, 5, and 8, MW markers in kDa (Invitrogen); lanes 2 & 3, hDM-αH-C6.5 MH3B1 at 1 and 2 μg, respectively, not reduced; lane 6 & 7, hDM-αH-C6.5 MH3B1 at 1 and 2 μg, respectively, reduced. (C), Size exclusion chromatography of hDM-αH-C6.5 MH3B1 under non-reducing condition using a Sepharose-6 column. For comparison, molecular weight standards were analyzed under identical conditions.