Figure 2

Enzymatic activity of hDM-αH-C6.5 MH3B1. (A), Lineweaver-Burk plot of enzyme activity of hDM-αH-C6.5 MH3B1 with F-dAdo as substrate. Conversion of F-dAdo to F-Ade was followed spectrophotometrically in real time by the increase in absorbance at 280 nm. Concentration of F-dAdo is in μM and v is based on mili-units of absorbance/min. (B), Proliferation of CT26 and CT26HER2/neu cells and (C), MCF-7HER2 cells in the presence or absence of F-dAdo or hDM-αH-C6.5 MH3B1 was determined in 72 hours by MTS. (D), 0.2 μM of hPNP-αH-C6.5 MH3B1 was incubated with CT26HER2/neu or MCF-7 cells in the presence of 1.5 or 6 μM of F-dAdo respectively for 72 hours and cellular proliferation determined by MTS assay. Error bars for each graph represent standard deviation within each set of values.