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Table 2 Kinetic constants of hPNP-αH-C6 MH3B1 for guanosine as substrate.

From: Characterization of an engineered human purine nucleoside phosphorylase fused to an anti-her2/neu single chain Fv for use in ADEPT

 

K M

(μM)

K cat

(s-1)

k cat /K M

(M-1s-1)

hPNP-αH-C6 MH3B1

59 ± 10

60 ± 13

1.02 × 104