Characterization of α1,2-FT-transfected cell lines. (A) RT-PCR profiles of α1,2-FT mRNA in non- and α1,2-FT-transfected cells. M: DNA ladder marker (100-2000 bp). (B) Relative expression of α1,2-FT mRNA in non- and α1,2-FT-transfected cells (n = 3). The data was expressed as the intensity ratio of α1,2-FT to β-actin (Mean ± SD). * p < 0.01 compared to the control. "A" is the representative of three independent and reproducible experiments. (C) Immunohistochemical staining for Lewis y antigen. (a) RMG-I-H cells; (b) RMG-I-pcDNA3.1 cells; (c) RMG-I cells; (d) RMG-I-H-A cells; (e) RMG-I-A cells. Meanwhile, a, b and c represents cells without α-L-fucosidase treatmeant; d and e represents cells with α-L-fucosidase treatmeant.