Figure 6

PI3K/Akt signaling is required for Lewis y-enhanced growth of RMG-I cells. (A) Western blot profiles of Akt and p-Akt in non- and α1,2-FT transfected cells, as well as in the absence and presence of anti-Lewis y antibody and LY294002. (B) Densitometric quantification of protein expression of A (n = 3). (C) Western blot profiles of PCNA in non- and α1,2-FT transfected cells, as well as in the absence and presence of anti-Lewis y antibody and LY294002. (D) Densitometric quantification of protein expression of C (n = 3).* p < 0.01 compared to RMG-I. # p < 0.01 compared to RMG-I-H cells without anti-Lewis y antibody or LY294002 treatment. "A" and "C" are the representative of three independent and reproducible experiments.