Figure 3

proliferation and apoptosis analysis by MTT assay and flow cytomerty. (A) shows that proliferation of LO2 cells expression shRNA. Proliferation of shRNA-transfected LO2 cells (clone 3), shRNA scramble control and un-transfected LO2 cells were analyzed by MTT assay as described earlier. The mean ± SE of three independent experiments are shown. LO2 cells transfeced with pGenesil-CENPE vector proliferation slowed. (B) the result of flow cytometry showed that the percent of apoptosis cells of LO2 cells transfected with pGenesil-CENPE vector is higher than cells transfected with scrambler control shRNA vector or mock.