Figure 3

Migration and invasion of Eahy926 and A549 cells with transwell chambers in vitro. (A) Cell migration was evaluated by Milliwell assays. Cells migrating to the lower surface of filters were stained with hematoxylin solution. Representative images of Eahy926 and A549 cells on the lower side of a membrane after incubation for 6 h; (B) The difference in migration ability between Eahy926 and A549 cells; Columns, mean of independent experiments measured in triplicate and repeated for three independent times; bars, SD (P < 0.01); (C) Invasion assay was conducted by using invasion chambers. Representative images of Eahy926 and A549 cells on the lower side of a membrane after incubation for 16 h; (D) The difference in invasion capacity between Eahy926 and A549 cells. Columns, mean of independent experiments measured in triplicate and repeated for three independent times; bars, SD (P < 0.01).