Figure 4

RASSF1A-mediated growth inhibition and cell death is enhanced by K-RasG12V. CNE-2 cells were transiently transfected with RASSF1A ± activated K-Ras. Trypan blue was added in situ after 48 h, and the dye uptake was quantitated. (a) Transfect efficiency of RASSF1A and K-RasG12V is confirmed by RT-PCR and western-blot. B: blank group, V: empty vector group, E: experimental group;(b) Cell death assays; up-panel: CNE-2 cells were transfected with RASSF1A ± K-RasG12V, phase contrast microscopic digital images were taken at 48 h post-transfection, RASSF1A promoted a modest growth inhibition that was enhanced by the presence of activated K-RasG12V; lower-panel: Trypan blue in situ staining, the dye uptake was enhanced when RASSF1A was co-expressed with activated K-Ras.