Figure 3

To investigate the cells deal with cytokines in vitro. A-B.) Morphology shows that TGF-β induced a rapid proliferation of cells, and cells presented a spindle-like shape. The IFN-γ group presented a reduction tendency on cell adhesion, the shape of cells present suspended or polygonal, lose normal B16 cells morphousorm. When given TGF-β and IFN-γ at the same time, cells returned to normal B16 cell shape, and cells also grew. C.) The results by wound healing assay showed that TGF-β confronting IFN-γ can promote migration. To treat cells only by IFN-γ inhibited cells migration. D.) Based on the Transwell invasion assay, IFN can inhibit cell migration, and inhibit cell invasion through Matrigel, and TGF-β has the opposite effect on cells to IFN-γ, and may have also an activity for inhibiting the IFN-γ activity, so that the cells migrate and invade.