Figure 4

To verify whether TGF-β and IFN-γ can enhance melanoma cells invasion by gelatin zymography assay analyzed in vitro and in vivo. A.) B16 cells treated by cytokines, show that IFN-γ can reduce the activity of MMP-2 and MMP-9, which are key modulators of tumor invasion. On the other hand, TGF-β can enhance the activity of both MMP-2 and MMP-9, giving TGF-β and IFN-γ. At the same time, TGF-β confronted IFN-γ to recover the activity of MMPs, and performed increasing activities on MMP-2 and MMP-9. B.) In vivo animal experiments also showed that there are significant features in day 7; the wound group had significantly lower activities of MMP-2 and MMP-9 compared with the control group from, 30% to 50%, respectively. By day 11, there was no significant difference in the activity of MMP-2 and MMP-9 between the wound groups and the control group. (*, p < 0.01)