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Table 1 The oligonucleotide primer sequences and PCR conditions for ALP, OP, and OC in this study.

From: Establishment of a new human osteosarcoma cell line, UTOS-1: cytogenetic characterization by array comparative genomic hybridization

Molecule

Primers

(5' to 3')

Strand

Size (bp)

Conditions

(temperature, cycle number)

ALP

ACGTGGCTAAGAATGTCATC

CTGGTAGGCGATGTCCTTA

+

--

475

55°C

35 cycles

OP

CCAAGTAAGTCCAACGAAAG

GGTGATGTCCTCGTCTGTA

+

--

347

58°C

45 cycles

OC

ATGAGAGCCCTCACACTCCTC

GCCGTAGAAGCGCCGATAGGC

+

--

294

59°C

45 cycles

GAPDH

GAAGGTGAAGGTCGGAGTCA

GAAGATGGTGATGGGATTTC

+

--

226

55°C

35 cycle

  1. Abbreviations: ALP, alkaline phosphatase; OP, osteopontin; OC, osteocalcin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.