Figure 2

Hypoxia induces expression of HIF-1α and survivin. A549 cells were cultured in 10% FBS medium under hypoxic or normoxic conditions for 24h. The relative levels of survivin (A) and HIF-1α (B) to GAPDH mRNA were determined by quantitative real time, reverse transcription-PCR. C: The expression of survivin and HIF-1α protein in A549 cells following HIF-1α-siRNA treatment as detected by Western blot analysis. D: The graph shows the statistical results of relative expression level of survivin and HIF-1α to β-actin protein. Data are given as means ± SD, n = 3, ** p < 0.01.