Figure 2

Western blot assay for the expression of pErk 1 and 2 and total Erk 1 and 2. The cells were processed for the determination of the phosphorylation and expression of Erk-1 and 2 evaluated after blotting with a specific anti-pMAPK and an anti-MAPK Mab, respectively, as described in "Materials and Methods". Expression of the house-keeping protein α-tubulin was used as loading control. In the same figure, the scores of the staining intensities of pErk-1 obtained at ICC in the same samples are also shown. The experiments were performed at least three different times and the results were always similar.