Figure 3

Effect of HPV-16 E5 expression on intracellular pH in FRM and M14 melanoma cells. Cells infected with the control retrovirus (CTR), cells treated with 20 nM Con-A (+ ConA) or cells expressing the HPV-16 E5 (+ E5), were stained with AO as described. The loss of orange fluorescence and the appearance of green fluorescence in cells treated with ConA or expressing E5 indicate the alkalinisation of endocellular organelles. A representative experiment in a set of four.