Overexpression of transketolase-like gene 1 is associated with cell proliferation in uterine cervix cancer

Table 1 The relative change in expression of transketolase gene family members in the human uterine cervix cancer and normal cervical epithelial cells

	HeLa cells	HeLa cells	HeLa cells	End1/E6E7 cells	End1/E6E7 cells
Gene	(No transfection)	(control siRNA)	(siRNA)	(control siRNA)	(siRNA)
TKT	1.05 ± 0.12	0.98 ± 0.09	1.06 ± 0.11	0.96 ± 0.10	1.02 ± 0.08
TKTL1	8.62 ± 0.92	8.43 ± 0.78	0.15 ± 0.02	1.03 ± 0.11	0.17 ± 0.03
TKTL 2	0.89 ± 0.10	1.12 ± 0.13	1.06 ± 0.11	0.99 ± 0.07	1.02 ± 0.09

The expression level of transketolase gene family members was analyzed using real-time quantitative PCR. The relative expression amount of target gene in the HeLa and End1/E6E7 cells was calculated using the 2 ^-ΔΔCT method. The relative expression amount of target gene in the HeLa cell was normalized to β-actin and relative to the expression of End1/E6E7 cells untreated. The relative expression amount of target gene in the End1/E6E7 cells (transfected with control plasmid and transfected with siRNA) was normalized to β-actin and relative to the expression of cells untreated.

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