Figure 5

Dynamics of multinucleation by time-lapse video microscopy. The interval of each image is 15 minutes. The yellow area indicates the location of the nuclei. In prophase, the nuclear body and the nuclear membrane were disaggregated and could not be seen (a-d), and in metaphase, the chromosomes were aggregated and could be seen in the equator of the cell (e). In anaphase, the daughter chromosomes separated, and in telophase the cytoplasm had an elongated shape, the cleavage furrow started to appear, and the nuclear membranes emerged and the cytoplasm began to constrict in the middle (f). However, the constriction stopped and reversed in the middle of cytokinesis, and the cytoplasm was not divided. As a result, the cell, which included two nuclei, was found in one cytoplasm (g-i).