Figure 2

displays the expression of GFP in HCT116 cells 48 h after transfected by Ad-GFP with different MOIs under fluorescent microscope at 200× magnification. The number of GFP-expressing cells increases in a MOI-dependent manner. When the MOI is more than 20, the infected cells still display bright green fluorescence, but their morphologies changes dramatically with less vigorously growing.