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Table 1 The parameters of SPEF used in SKOV3 cell suspensions.

From: The effect of high frequency steep pulsed electric fields on in vitro and in vivo antitumor efficiency of ovarian cancer cell line skov3 and potential use in electrochemotherapy

Test

group

Frequency

(Hz)

Intensity

(V/cm)

Rise time

(ns)

Duration

(μs)

Stimulation time

(minutes)

Group 1

1

50, 100, 150, 200, 250, 300, 350, 400

160

20

30

Group 2

60

50, 100, 150, 200, 250, 300, 350, 400

160

20

30

Group 3

1 000

50, 100, 150, 200, 250, 300, 350, 400

160

20

30

Group 4

5 000

50, 100, 150, 200, 250, 300, 350, 400

160

20

30

  1. In the first procedure, each intensity constituted a separate experiment contained in a certain test group, and cell exposure time was 30 minutes for each intensity corresponding to a given frequency.