Western blot analisis of acetylated histone H3 in IEC-6 cells. Total protein (30 μg per lane) was resolved on SDS-polyacrylamide gel and transferred onto a nitrocellulose membrane and incubated with anti-acetylated histone H3 and anti-GAPDH antibodies. The bands were visualized by using the enhanced chemiluminescence system. The relative abundances of acetylated histone H3 was normalized by that of GAPDH. Lane1: normal IEC-6 cells; Lane2: IEC-6 cells treated with MNNG/PMA for 6 times; Lane2: IEC-6 cells treated with MNNG/PMA for 12 times.