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Figure 3 | Journal of Experimental & Clinical Cancer Research

Figure 3

From: Alpha6beta4 integrin crosslinking induces EGFR clustering and promotes EGF-mediated Rho activation in breast cancer

Figure 3

The effect of α6β4 crosslinking on EGFR signaling following treatment with EGF (A) or HB-EGF (B). A) MDA-MB-231 cells in suspension were exposed to anti-β4 on ice, followed by control rabbit IgG (lanes 1 and 3) or rabbit anti-mouse IgG (lanes 2 and 4) at 37°C for 30 min to crosslink α6β4, with (lanes 3 and 4) or without (lanes 1 and 2) subsequent addition of EGF (10 ng/ml) for 5 min. B) MDA-MB-231 cells were exposed to anti-β4 on ice, then added to plates coated with control rabbit IgG (lanes 1, 3, 5, 7, 9 and 11) or rabbit anti-mouse IgG (lanes 2, 4, 6, 8, 10, or 12) at 37°C to crosslink α6β4, in the presence (lanes 3, 4, 7, 8, 11, and 12) or absence(lanes 1, 2, 5, 6, 9, and 10) of simultaneous coating with HB-EGF. Western blots prepared from cell lysates were probed for phospho-Akt and phospho-Erk1,2, then stripped and probed for total Akt and total Erk1,2.

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